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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
23/10/2015 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LADO, J.; RODRIGO, M.J.; LÓPEZ-CLIMENT, M.; GÓMEZ-CADENAS, A.; ZACARÍAS, L. |
Afiliación : |
JOANNA LADO LINDNER, IATA (Instituto de Agroquímica y Tecnología de Alimentos); INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JESÚS RODRIGO, IATA (Instituto de Agroquímica y Tecnología de Alimentos); MARÍA LÓPEZ-CLIMENT, Universidad Jaume I de Castellón (ES); AURELIO GÓMEZ-CADENAS, Universidad Jaume I de Castellón (ES); LORENZO ZACARÍAS, IATA (Instituto de Agroquímica y Tecnología de Alimentos). |
Título : |
Implication of the antioxidant system in chilling injury tolerance in the red peel of grapefruit. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Postharvest Biology and Technology, 2016, 111 , art. no. 10121 , pp. 214 - 223 . |
DOI : |
10.1016/j.postharvbio.2015.09.013 |
Idioma : |
Inglés |
Notas : |
Article history: Received 20 February 2015 / Received in revised form 10 September 2015 / Accepted 10 September 2015. |
Contenido : |
ABSTRACT.
Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star
Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with
enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay revealed considerably higher activity in the lycopene-accumulating peel than in the yellow peel at harvest time and throughout the entire cold storage and shelf-life period. Enzymatic activity and gene expression analyses of GR, APX and SOD did not reveal the involvement of these antioxidant enzymes in the protection against CI. However, high CAT activity was detected in the peel of CI-tolerant fruit, although this difference did not correlate with changes in the expression levels of the CAT1 and CAT2 genes. Therefore, the boost in singlet oxygen scavenging capacity is likely the primary factor responsible for CI tolerance in the lycopene-accumulating peel of grapefruit.
@ 2015 Elsevier B.V. All rights reserved. MenosABSTRACT.
Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star
Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with
enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay reveale... Presentar Todo |
Thesagro : |
ANTIOXIDANTES; CITRUS; DANOS POR HELADA; LYCOPENE. |
Asunto categoría : |
-- |
Marc : |
LEADER 03039naa a2200241 a 4500 001 1053719 005 2019-10-15 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.postharvbio.2015.09.013$2DOI 100 1 $aLADO, J. 245 $aImplication of the antioxidant system in chilling injury tolerance in the red peel of grapefruit. 260 $c2015 500 $aArticle history: Received 20 February 2015 / Received in revised form 10 September 2015 / Accepted 10 September 2015. 520 $aABSTRACT. Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay revealed considerably higher activity in the lycopene-accumulating peel than in the yellow peel at harvest time and throughout the entire cold storage and shelf-life period. Enzymatic activity and gene expression analyses of GR, APX and SOD did not reveal the involvement of these antioxidant enzymes in the protection against CI. However, high CAT activity was detected in the peel of CI-tolerant fruit, although this difference did not correlate with changes in the expression levels of the CAT1 and CAT2 genes. Therefore, the boost in singlet oxygen scavenging capacity is likely the primary factor responsible for CI tolerance in the lycopene-accumulating peel of grapefruit. @ 2015 Elsevier B.V. All rights reserved. 650 $aANTIOXIDANTES 650 $aCITRUS 650 $aDANOS POR HELADA 650 $aLYCOPENE 700 1 $aRODRIGO, M.J. 700 1 $aLÓPEZ-CLIMENT, M. 700 1 $aGÓMEZ-CADENAS, A. 700 1 $aZACARÍAS, L. 773 $tPostharvest Biology and Technology, 2016, 111 , art. no. 10121 , pp. 214 - 223 .
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
02/12/2015 |
Actualizado : |
29/05/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
GARCÍA ROCHE, M.; PEREZ, E. |
Afiliación : |
MERCEDES GARCÍA ROCHE, Universidad ORT Uruguay, Licenciatura en Biotecnología, Trabajo final de Grado.; ELENA PEREZ FAGGIANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Aceites esenciales de plantas nativas promisorios para el control de bacteriosis en tomate. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resultados experimentales en sanidad de tomate y morrón. Jornada de Divulgación. Canelones (Uruguay): INIA, 2015. |
Páginas : |
p. 62-65 |
Serie : |
(Serie Actividades de Difusión ; 756). |
ISSN : |
1688-9258. |
Idioma : |
Español |
Notas : |
La investigación que da origen a los resultados presentados en la presente publicación recibió fondos de la Agencia Nacional de Investigación e Innovación bajo el código
INI_X_2013_1_10160 y del Instituto Nacional de Investigación Agropecuaria fondos 50 proyecto N 12827 HO-16_0_0. |
Contenido : |
El objetivo del presente trabajo fue conocer el efecto de 15 aceites esenciales (a.e.) obtenidos de plantas nativas de Uruguay, sobre el control de las bacterias Xanthomonas gardneri,
Xanthomonas vesicatoria, Pseudomonas syringae y Clavibacter michiganensis subsp. michiganensis que afectan a Solanum lycopersicum L. (tomate ) y contar con una primera
aproximación a la dosis efectiva para evaluaciones en plantas. |
Thesagro : |
BACTERIOSIS; CONTROL; TOMATE. |
Asunto categoría : |
-- H20 Enfermedades de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9849/1/sad-756-p.62-65.pdf
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Marc : |
LEADER 01468naa a2200217 a 4500 001 1054056 005 2018-05-29 008 2015 bl uuuu u00u1 u #d 022 $a1688-9258. 100 1 $aGARCÍA ROCHE, M. 245 $aAceites esenciales de plantas nativas promisorios para el control de bacteriosis en tomate. 260 $c2015 300 $ap. 62-65 490 $a(Serie Actividades de Difusión ; 756). 500 $aLa investigación que da origen a los resultados presentados en la presente publicación recibió fondos de la Agencia Nacional de Investigación e Innovación bajo el código INI_X_2013_1_10160 y del Instituto Nacional de Investigación Agropecuaria fondos 50 proyecto N 12827 HO-16_0_0. 520 $aEl objetivo del presente trabajo fue conocer el efecto de 15 aceites esenciales (a.e.) obtenidos de plantas nativas de Uruguay, sobre el control de las bacterias Xanthomonas gardneri, Xanthomonas vesicatoria, Pseudomonas syringae y Clavibacter michiganensis subsp. michiganensis que afectan a Solanum lycopersicum L. (tomate ) y contar con una primera aproximación a la dosis efectiva para evaluaciones en plantas. 650 $aBACTERIOSIS 650 $aCONTROL 650 $aTOMATE 700 1 $aPEREZ, E. 773 $tIn: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resultados experimentales en sanidad de tomate y morrón. Jornada de Divulgación. Canelones (Uruguay): INIA, 2015.
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